3NGH
Molecular Analysis of the Interaction of the HDL Receptor SR-BI with the Adaptor Protein PDZK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.604, 55.114, 111.055 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.910 - 1.800 |
| R-factor | 0.20158 |
| Rwork | 0.200 |
| R-free | 0.23763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2edz |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.851 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 1.860 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.488 | 0.055 |
| Number of reflections | 26518 | |
| <I/σ(I)> | 41.1 | 3.7 |
| Completeness [%] | 97.5 | 91.7 |
| Redundancy | 10.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100mM Tris/HCl, 40% PEG 200, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
