3NGF
Crystal structure of AP endonuclease, family 2 from Brucella melitensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-11 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.980, 120.640, 54.680 |
| Unit cell angles | 90.00, 117.14, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.800 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k77 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | |
| Number of reflections | 55966 | |
| <I/σ(I)> | 14.46 | 3.6 |
| Completeness [%] | 98.8 | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 0.04 M KH2PO4, 16% PEG 8000, 20% GLYCEROL, 27 MG/ML PROTEIN, JCSG+ SCREEN WELL D12, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
