3NG2
Crystal structure of the RNF4 ring domain dimer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2009-07-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.2829, 1.2833, 1.2523 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.487, 85.922, 22.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.730 - 1.800 |
| R-factor | 0.19001 |
| Rwork | 0.187 |
| R-free | 0.24199 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.546 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.010 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.274 |
| Number of reflections | 10936 | |
| <I/σ(I)> | 16.1 | 6.7 |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 0.2M lithium sulfate, 0.1M bis-Tris, 22% PEG 3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
