3NG0
Crystal Structure of Glutamine Synthetase from Synechocystis sp. PCC 6803
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 132.016, 132.016, 202.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.920 - 2.800 |
R-factor | 0.2867 |
Rwork | 0.285 |
R-free | 0.31290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.160 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.213 | 0.157 | 0.424 |
Number of reflections | 25802 | ||
<I/σ(I)> | 4.1 | ||
Completeness [%] | 98.0 | 93.4 | 99.2 |
Redundancy | 6.7 | 6.3 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1M sodium acetate,0.1M calcium acetate, 10% PEG 4000, pH 4.0, vapor diffusion, hanging drop, temperature 298K |