3NFI
Crystal structure of tandem winged helix domain of RNA polymerase I subunit A49
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2009-05-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790, 0.9790, 0.9180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.110, 78.100, 100.670 |
| Unit cell angles | 90.00, 113.39, 90.00 |
Refinement procedure
| Resolution | 27.820 - 1.900 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.22350 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.080 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.606 |
| Number of reflections | 94313 | |
| <I/σ(I)> | 14.78 | 3.4 |
| Completeness [%] | 97.5 | 99.8 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 24% PEG 3350, 50 mM Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
