3NFI
Crystal structure of tandem winged helix domain of RNA polymerase I subunit A49
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2009-05-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9790, 0.9790, 0.9180 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.110, 78.100, 100.670 |
Unit cell angles | 90.00, 113.39, 90.00 |
Refinement procedure
Resolution | 27.820 - 1.900 |
R-factor | 0.1903 |
Rwork | 0.189 |
R-free | 0.22350 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.080 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.066 | 0.606 |
Number of reflections | 94313 | |
<I/σ(I)> | 14.78 | 3.4 |
Completeness [%] | 97.5 | 99.8 |
Redundancy | 3.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 24% PEG 3350, 50 mM Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |