3NFH

Crystal structure of tandem winged helix domain of RNA polymerase I subunit A49 (P4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	70
Detector technology	CCD
Collection date	2009-05-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	P 43
Unit cell lengths	96.710, 96.710, 54.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.200 - 2.170
R-factor	0.1897
Rwork	0.188
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Crystal structure of tandem winged helix domain of RNA polymerase I subunit A49 (SG P21 on hold)
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	2.450
High resolution limit [Å]	2.170	2.170
Rmerge	0.080	0.597
Number of reflections	26581
<I/σ(I)>	27.5	6.3
Completeness [%]	99.8	99.8
Redundancy	11.7	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	26% PEG 4000, 200 mM lithium sulfate monohydrate, 100 mM Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K