3NFG
Crystal structure of Dimerization module of RNA polymerase I subcomplex A49/A34.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Collection date | 2008-06-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.426, 132.920, 118.286 |
| Unit cell angles | 90.00, 102.84, 90.00 |
Refinement procedure
| Resolution | 49.680 - 2.510 |
| R-factor | 0.1966 |
| Rwork | 0.194 |
| R-free | 0.24340 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.510 | 2.500 |
| Rmerge | 0.037 | 0.651 |
| Number of reflections | 88325 | |
| <I/σ(I)> | 15.61 | 3.3 |
| Completeness [%] | 97.8 | 97.6 |
| Redundancy | 4.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22% PEG 3350, 250 mM sodium flouride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
