3NFF
Crystal structure of extended Dimerization module of RNA polymerase I subcomplex A49/A34.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2008-12-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9330 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 108.930, 221.770, 129.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.100 - 3.240 |
R-factor | 0.2904 |
Rwork | 0.287 |
R-free | 0.36080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Crystal structure of Dimerization module of RNA polymerase I subcomplex A49/A34.5 (SG P 21) |
RMSD bond length | 0.010 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 3.380 |
High resolution limit [Å] | 3.240 | 3.240 |
Rmerge | 0.207 | 0.792 |
Number of reflections | 25171 | |
<I/σ(I)> | 5.58 | 3.7 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 8 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 50 mM Tris pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |