3NF5
Crystal structure of the C-terminal domain of nuclear pore complex component NUP116 from Candida glabrata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.703, 67.668, 55.119 |
| Unit cell angles | 90.00, 101.37, 90.00 |
Refinement procedure
| Resolution | 25.090 - 1.940 |
| R-factor | 0.20924 |
| Rwork | 0.207 |
| R-free | 0.25617 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Poly-alanine model of yeast Nup145 PDB ID: 3KEP |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.781 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.370 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 25957 | |
| <I/σ(I)> | 8.3 | 3.6 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 294 | 100mM MES, 25% PEGMME 2000, 200mM Sodium potassium tartarae, 0.4microL of 5% ethylacetate used additive into 2microL drop, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
