3NEU
The crystal structure of a functionally-unknown protein lin1836 from Listeria innocua Clip11262
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 41.828, 88.051, 38.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.264 - 1.580 |
R-factor | 0.1951 |
Rwork | 0.194 |
R-free | 0.22190 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.966 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.500 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.092 | 0.747 |
Number of reflections | 19919 | |
<I/σ(I)> | 38 | 2.1 |
Completeness [%] | 98.9 | 100 |
Redundancy | 4.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 10%(w/v)PEG1000, 10%(w/v)PEG8000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |