3NEU
The crystal structure of a functionally-unknown protein lin1836 from Listeria innocua Clip11262
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 41.828, 88.051, 38.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.264 - 1.580 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.22190 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.966 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.092 | 0.747 |
| Number of reflections | 19919 | |
| <I/σ(I)> | 38 | 2.1 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 10%(w/v)PEG1000, 10%(w/v)PEG8000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
