3NER
Structure of Human Type B Cytochrome b5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-08 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.316, 40.034, 58.744 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 29.240 - 1.450 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lj0 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.240 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.057 | 0.453 |
| Number of reflections | 29891 | |
| <I/σ(I)> | 18.45 | 2.13 |
| Completeness [%] | 97.6 | |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 278 | 30% PEG8K, 0.1M Hepes (pH 6.8) and 0.2M magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
