3NEN

Unliganded aspartyl-tRNA synthetase from thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	120
Detector technology	CCD
Collection date	1996-12-10
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 2
Unit cell lengths	124.100, 125.100, 87.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.270 - 2.400
R-factor	0.211
Rwork	0.211
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1asz
RMSD bond length	0.008
RMSD bond angle	1.530
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.268
High resolution limit [Å]	2.400
Number of reflections	48410
Completeness [%]	89.9
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			297	peg and ethylen glycol, VAPOR DIFFUSION, HANGING DROP, temperature 297K