3NEK
Crystal structure of a nitrogen repressor-like protein MJ0159 from Methanococcus jannaschii
Replaces: 2QYXExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 80.009, 80.009, 116.421 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.150 - 2.500 |
R-factor | 0.247 |
Rwork | 0.246 |
R-free | 0.27700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.116 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.075 | 0.590 |
Number of reflections | 15322 | |
<I/σ(I)> | 7.1 | 1 |
Completeness [%] | 99.2 | 98.8 |
Redundancy | 4.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | 100mM sodium MES pH 7.0, 40% MPD, vapor diffusion, temperature 294K |