3NE6
RB69 DNA Polymerase (S565G/Y567A) Ternary Complex with dCTP Opposite dG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.10000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.135, 119.783, 130.652 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.300 - 2.001 |
R-factor | 0.18562 |
Rwork | 0.184 |
R-free | 0.22568 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nci |
RMSD bond length | 0.007 |
RMSD bond angle | 1.055 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 0.781 |
Number of reflections | 79966 | |
<I/σ(I)> | 18.1 | 1.6 |
Completeness [%] | 99.7 | 97.8 |
Redundancy | 4.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 293 | 7% (w/v) PEG 350 monomethyl ether (MME), 175 mM CaCl2, 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K |