3NE3
Mycobacterium tuberculosis Acyl Carrier Protein Synthase Apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2000-01-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 67.933, 67.933, 83.982 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.190 - 1.900 |
R-factor | 0.249 |
Rwork | 0.248 |
R-free | 0.27400 |
RMSD bond length | 0.036 |
RMSD bond angle | 2.649 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.190 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.030 |
Number of reflections | 10843 |
<I/σ(I)> | 54 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.6% PEG 4K, 0.07 M sodium acetate pH 4.6 to 5.5, 30% glycerol, EVAPORATION |