3NE0
Structure and functional regulation of RipA, a mycobacterial enzyme essential for daughter cell separation
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9737, 0.9785, 0.9787, 0.9737 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.866, 65.560, 67.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.000 |
| R-factor | 0.1055 |
| Rwork | 0.104 |
| R-free | 0.13700 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.080 | 0.292 |
| Number of reflections | 85882 | |
| <I/σ(I)> | 19.7 | 3 |
| Completeness [%] | 96.5 | 72.6 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 293 | 8% (v/v) 2-Propanol, 16% (w/v) PEG4000, 60 mM Sodium citrate trihydrate buffer, pH 5.6, EVAPORATION, temperature 293K |
