3ND9
Structural characterization for the nucleotide binding ability of subunit A of the A1AO ATP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.381, 128.381, 105.057 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 3.100 |
R-factor | 0.22303 |
Rwork | 0.220 |
R-free | 0.28531 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vdz |
RMSD bond length | 0.015 |
RMSD bond angle | 1.738 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 3.100 |
Number of reflections | 16262 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 50% (v/v) MPD, 0.1 M acetate (pH 4.5), vapor diffusion, hanging drop , temperature 291K |