3ND3

Uhelix 16-mer dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9184
Spacegroup name	H 3 2
Unit cell lengths	42.735, 42.735, 126.358
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.309 - 1.370
R-factor	0.187
Rwork	0.182
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.330
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.309	19.060	1.440
High resolution limit [Å]	1.370	4.330	1.370
Rmerge		0.019	0.503
Total number of observations		5965	17829
Number of reflections	9679
<I/σ(I)>	52	26.2	1.6
Completeness [%]	99.8	98.6	99.1
Redundancy	17.2	17.3	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	50 mM NaCacodylate pH 6.0, 10 mM MgCl2, and 1 M LiSO4, vapor diffusion, hanging drop, temperature 298K