3ND3
Uhelix 16-mer dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 42.735, 42.735, 126.358 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.309 - 1.370 |
| R-factor | 0.187 |
| Rwork | 0.182 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 18.309 | 19.060 | 1.440 |
| High resolution limit [Å] | 1.370 | 4.330 | 1.370 |
| Rmerge | 0.019 | 0.503 | |
| Total number of observations | 5965 | 17829 | |
| Number of reflections | 9679 | ||
| <I/σ(I)> | 52 | 26.2 | 1.6 |
| Completeness [%] | 99.8 | 98.6 | 99.1 |
| Redundancy | 17.2 | 17.3 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50 mM NaCacodylate pH 6.0, 10 mM MgCl2, and 1 M LiSO4, vapor diffusion, hanging drop, temperature 298K |
