3ND2
Structure of Yeast Importin-beta (Kap95p)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-03 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.835, 128.272, 69.050 |
| Unit cell angles | 90.00, 102.68, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.400 |
| R-factor | 0.21523 |
| Rwork | 0.213 |
| R-free | 0.26668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bku |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.243 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.075 |
| Number of reflections | 37902 |
| <I/σ(I)> | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 4000, 0.1M MES pH 6.5, 20mM MgCl2, 125mM NaCl, 12% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
