3NCT

X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of reca

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97919
Spacegroup name	P 43 21 2
Unit cell lengths	93.815, 93.815, 164.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.18619
Rwork	0.185
R-free	0.21029
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.059
Number of reflections	71325
<I/σ(I)>	22
Completeness [%]	99.8	100
Redundancy	6	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	298	136 mM sodium acetate, pH 5.2, 1.4% PEG 4000, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K