3NCQ
GlnK2 from Archaeoglobus fulgidus, ATP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 121.663, 121.663, 124.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.300 - 1.240 |
| R-factor | 0.16968 |
| Rwork | 0.168 |
| R-free | 0.20837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ncp |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.403 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 1.340 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.108 | 0.589 |
| Number of reflections | 130294 | |
| <I/σ(I)> | 9.93 | 2.7 |
| Completeness [%] | 99.2 | 96.5 |
| Redundancy | 6.54 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | ammonium phosphate, Tris-HCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
