3NCI
RB69 DNA Polymerase Ternary Complex with dCTP Opposite dG at 1.8 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9095 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.504, 119.404, 129.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.790 |
| R-factor | 0.17607 |
| Rwork | 0.175 |
| R-free | 0.20136 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ig9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.077 | 0.813 |
| Number of reflections | 99370 | |
| <I/σ(I)> | 13.7 | 0.86 |
| Completeness [%] | 94.9 | 74 |
| Redundancy | 3.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-batch vapor-diffusion | 6.5 | 293 | 8%(w/v) PEG 350 monomethyl ether (MME),160 mM CaCl2, and 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K |
