3NCF
A mutant human Prolactin receptor antagonist H30A in complex with the mutant extracellular domain H188A of the human prolactin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.252, 123.252, 73.108 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.990 - 2.800 |
| R-factor | 0.16039 |
| Rwork | 0.157 |
| R-free | 0.21946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mzg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.890 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.164 | 0.082 | 0.767 |
| Number of reflections | 21573 | ||
| <I/σ(I)> | 7 | ||
| Completeness [%] | 97.8 | 95.7 | 97.6 |
| Redundancy | 7 | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 3.6M NaCl, 0.1 M Hepes 7.5, 3% monomethylether 550, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
