3NC1
Crystal structure of the CRM1-RanGTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 156.802, 216.158, 123.818 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.521 - 3.350 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRM1 and RANGTP molecules taken from PDB entry 3GJX |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.528 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.370 | 3.450 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.052 | 0.500 |
| Number of reflections | 28804 | 1679 |
| <I/σ(I)> | 18.06 | 2.1 |
| Completeness [%] | 94.1 | 66.4 |
| Redundancy | 4.12 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Tris/HCl, 12% (w/v) PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
