3NBX
Crystal structure of E. coli RavA (Regulatory ATPase variant A) in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 65 |
| Unit cell lengths | 162.232, 162.232, 55.316 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.700 - 2.910 |
| R-factor | 0.22574 |
| Rwork | 0.223 |
| R-free | 0.26923 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 3.040 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.065 | |
| Number of reflections | 18402 | |
| <I/σ(I)> | 25 | 2.9 |
| Completeness [%] | 99.2 | 95.6 |
| Redundancy | 5.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 2 mM ATP, 10 mM MgCl2, 0.1-0.6 M ammonium sulfate, 10-20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
