3NBB
Crystal structure of mutant Y305F expressed in E. coli in the copper amine oxidase from hansenula polymorpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-05 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.523, 232.618, 104.146 |
Unit cell angles | 90.00, 91.51, 90.00 |
Refinement procedure
Resolution | 36.130 - 2.050 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a2v |
RMSD bond length | 0.006 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP (from ccp4) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.067 | 0.318 |
Number of reflections | 217301 | |
<I/σ(I)> | 14.2 | 3.3 |
Completeness [%] | 77.4 | |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 295 | 20% PEG8000, 100mM HEPES, 2% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |