Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

3NAV

Crystal structure of an alpha subunit of tryptophan synthase from Vibrio cholerae O1 biovar El Tor str. N16961

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]100
Detector technologyCCD
Collection date2010-05-18
DetectorADSC QUANTUM 315r
Wavelength(s)0.9794
Spacegroup nameP 21 21 21
Unit cell lengths57.594, 60.380, 141.197
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution40.000 - 2.100
R-factor0.18201
Rwork0.179
R-free0.23304
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1xc4
RMSD bond length0.020
RMSD bond angle1.668
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0002.140
High resolution limit [Å]2.1002.100
Rmerge0.0910.380
Number of reflections29864
<I/σ(I)>108.3
Completeness [%]99.9100
Redundancy5.35.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP72910.2 M Ammonium flouride 20% Peg 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K

227933

PDB entries from 2024-11-27

PDB statisticsPDBj update infoContact PDBjnumon