3NAT
Crystal Structure of Conserved Protein of Unknown Function EF_1977 from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.381, 81.381, 164.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.691 - 2.925 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.913 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.030 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Number of reflections | 12505 | |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 99.4 | 95.6 |
| Redundancy | 16.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 1.0 M Sodium Citrate, 0.1 M Imidazole pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
