3NAF
Structure of the Intracellular Gating Ring from the Human High-conductance Ca2+ gated K+ Channel (BK Channel)
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-03-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 134.395, 134.395, 231.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.483 - 3.100 |
R-factor | 0.2429 |
Rwork | 0.238 |
R-free | 0.28890 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.709 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.160 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.063 | 0.529 |
Number of reflections | 19761 | |
<I/σ(I)> | 30 | 2.2 |
Completeness [%] | 96.2 | 80 |
Redundancy | 8.8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 3.5M sodium formate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 3.5M sodium formate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |