3NA6
Crystal structure of a succinylglutamate desuccinylase (TM1040_2694) from SILICIBACTER SP. TM1040 at 2.00 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97922,0.97905 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 99.773, 99.773, 137.179 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.487 - 2.000 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.19500 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.513 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.487 | 29.487 | 2.070 |
High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
Rmerge | 0.157 | 0.013 | 0.013 |
Number of reflections | 27876 | 5232 | 4903 |
<I/σ(I)> | 11.56 | 39.1 | 1.8 |
Completeness [%] | 99.5 | 99.6 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2000M NaCl, 30.0000% PEG-3000, 0.1M TRIS pH 7.0, 0.006 M Calcium Chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |