3NA4
D53P beta-2 microglobulin mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 29.070, 50.730, 71.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.220 - 1.900 |
R-factor | 0.21702 |
Rwork | 0.211 |
R-free | 0.26865 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z9t |
RMSD bond length | 0.013 |
RMSD bond angle | 1.533 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.910 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.106 | 0.569 |
Number of reflections | 8702 | |
<I/σ(I)> | 8.4 | 2.4 |
Completeness [%] | 99.2 | 99 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 4000, Sodium Acetate, Glycerol, Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |