3NA2
Crystal Structure of Protein of Unknown Function from Mine Drainage Metagenome Leptospirillum rubarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-04 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97910 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 76.367, 115.047, 73.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.240 - 2.293 |
R-factor | 0.19 |
Rwork | 0.186 |
R-free | 0.24800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.975 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 29653 | |
<I/σ(I)> | 11.9 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.8 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Sodium Cacodylate pH 6.5, 0.2 M Magnesium acetate, 20 % PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |