3N9P
ceKDM7A from C.elegans, complex with H3K4me3K27me2 peptide and NOG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-06 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.2828 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.112, 86.368, 62.744 |
Unit cell angles | 90.00, 113.18, 90.00 |
Refinement procedure
Resolution | 31.478 - 2.388 |
R-factor | 0.2025 |
Rwork | 0.200 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n9m |
RMSD bond length | 0.008 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.388 | 2.388 |
Number of reflections | 23334 | |
Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 16% PEG 3350, 0.2M Sodium Fluoride, 0.1M Bis-Tris or HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |