3N9L
ceKDM7A from C.elegans, complex with H3K4me3 peptide and NOG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-06 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.2828 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.912, 87.264, 102.491 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.189 - 2.796 |
R-factor | 0.1999 |
Rwork | 0.197 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n9m |
RMSD bond length | 0.004 |
RMSD bond angle | 0.832 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.6.1_353)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.796 | 2.796 |
Total number of observations | 128353 | |
Number of reflections | 15846 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 16% PEG 3350, 0.2M Sodium Fluoride, 0.1M Bis-Tris/HEPES , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |