3N9H
Crystal Structural of mutant Y305A in the copper amine oxidase from hansenula polymorpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 138.910, 147.150, 233.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.100 - 2.500 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a2v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.100 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.224 |
| Number of reflections | 134330 | |
| <I/σ(I)> | 14.1 | 3.1 |
| Completeness [%] | 79.1 | 41 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 295 | 10% PEG8000. 0.125M Lithium sulfate and 0.1M KPi, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
