3N9C
Mite-y Lysozyme: Marmite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.414, 79.414, 38.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.290 - 1.500 |
| R-factor | 0.16186 |
| Rwork | 0.160 |
| R-free | 0.19015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2blx |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.428 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | 0.495 |
| Number of reflections | 19946 | |
| <I/σ(I)> | 19.9 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.5 | 293 | Crystals were obtained using counter diffusion using Marmite, pH 4.5, LIQUID DIFFUSION, temperature 293K |
