3N9A
Mite-y Lysozyme: Vegemite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.289, 79.289, 37.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.390 - 1.400 |
| R-factor | 0.16296 |
| Rwork | 0.162 |
| R-free | 0.18851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2blx |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.645 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.055 | 0.454 |
| Number of reflections | 23901 | |
| <I/σ(I)> | 35.7 | 6.1 |
| Completeness [%] | 98.0 | 87 |
| Redundancy | 18.6 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.5 | 293 | Crystals were obtained by counter diffusion in capillary tubes stuck into Vegemite., pH 4.5, LIQUID DIFFUSION, temperature 293K |
