3N8F
Crystal structure of the complex of goat lactoperoxidase with thiocyanate at 3.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.522, 72.537, 83.983 |
| Unit cell angles | 85.30, 84.06, 75.68 |
Refinement procedure
| Resolution | 24.980 - 3.250 |
| R-factor | 0.20954 |
| Rwork | 0.205 |
| R-free | 0.24827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ojv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.246 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 20901 | |
| <I/σ(I)> | 3.8 | 1.9 |
| Completeness [%] | 95.2 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 280 | 0.2M Ammonium nitrate 20%w/v PEG 3350, PH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
