3N8E
Substrate binding domain of the human Heat Shock 70kDa protein 9 (mortalin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 118.680, 118.680, 159.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.940 - 2.800 |
| R-factor | 0.2085 |
| Rwork | 0.206 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 16980 | |
| <I/σ(I)> | 5.82 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 41.9 | 43.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.2 M sodium chloride, 20% PEG 8000, 0.1 M citric acid, 0.1 M di-sodium hydrogen phosphate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
