3N7Z
Crystal structure of acetyltransferase from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.429, 176.859, 109.973 |
| Unit cell angles | 90.00, 105.73, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.750 |
| R-factor | 0.19005 |
| Rwork | 0.187 |
| R-free | 0.24238 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.149 | 0.929 |
| Number of reflections | 75594 | |
| <I/σ(I)> | 19.2 | 2.26 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1M sodium HEPES, 20% PEG3350, 0.2M sodium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
