3N7X
Crystal structure of Penaeus stylirostris densovirus capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 235.890, 245.471, 268.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.278 |
| Rwork | 0.278 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRYO-ELECTRON MICROSCOPY RECONSTRUCTION |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.412 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | envelope |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.107 | 0.598 |
| Number of reflections | 338268 | |
| <I/σ(I)> | 12.1 | 1.35 |
| Completeness [%] | 64.4 | 45.5 |
| Redundancy | 2.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5% PEG8000, 10mM Tris-HCl, 5% glycerol, starting protein concentration in drop 7mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
