3N7C
Crystal structure of the RAN binding domain from the nuclear pore complex component NUP2 from Ashbya gossypii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.540, 62.550, 76.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.170 - 2.260 |
R-factor | 0.236 |
Rwork | 0.234 |
R-free | 0.29300 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.774 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX (C) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.120 | 2.380 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 13396 | |
<I/σ(I)> | 15.7 | 5.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 13.5 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 100mM Bis-Tris pH 5.5 + 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |