3N6T
Effector binding domain of TsaR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97850 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.630, 94.580, 83.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.200 - 1.850 |
| R-factor | 0.22284 |
| Rwork | 0.220 |
| R-free | 0.27479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fxq |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.073 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.530 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.059 | 0.360 |
| Number of reflections | 19168 | |
| <I/σ(I)> | 10 | 2.68 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 1.83 | 1.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1.5M ammonium sulfate, 0.1M sodium acetate pH4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
