3N6O
Crystal structure of the GEF and P4M domain of DrrA/SidM from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.962, 75.375, 130.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.500 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.23600 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.651 |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.552 | |
| Number of reflections | 26358 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 298 | 20% (w/v) PEG4000, 0.16 M ammonium sulfate, 0.1 M sodium acetate, 20% glycerol, pH 4.8, vapor diffusion, hanging drop, temperature 298K |
