3N5J
Human fpps complex with NOV_311
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2004-09-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 111.112, 111.112, 78.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.950 - 2.350 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.24000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | APRV |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.050 | 0.191 |
| Number of reflections | 20763 | |
| <I/σ(I)> | 23.7 | 9.1 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 292 | 1.2M sodium potassium phosphate, 25% glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
