3N5J
Human fpps complex with NOV_311
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2004-09-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97936 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 111.112, 111.112, 78.245 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.950 - 2.350 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.24000 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | APRV |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.050 | 0.191 |
Number of reflections | 20763 | |
<I/σ(I)> | 23.7 | 9.1 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 292 | 1.2M sodium potassium phosphate, 25% glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |