3N5F
Crystal Structure of L-N-carbamoylase from Geobacillus stearothermophilus CECT43
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-03-03 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.208, 211.683, 43.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.750 |
| R-factor | 0.1968 |
| Rwork | 0.193 |
| R-free | 0.27579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2l |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.556 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.890 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.124 | 0.258 |
| Number of reflections | 25512 | |
| <I/σ(I)> | 15.05 | 4.16 |
| Completeness [%] | 99.1 | 91.5 |
| Redundancy | 7.26 | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 15 % iso-propanol, 0.1 M sodium cacodylate pH 6.5 and 0.6 M trisodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
