3N4Z

Crystal structure of quintuple Arg-to-Lys variant of T. celer L30e

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2009-01-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	30.487, 61.360, 102.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.489 - 2.397
R-factor	0.1887
Rwork	0.182
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h7m
RMSD bond length	0.007
RMSD bond angle	1.069
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.440	2.530
High resolution limit [Å]	2.397	2.397
Rmerge	0.126	0.268
Total number of observations	119186
Number of reflections	8086
<I/σ(I)>	15.4	8.7
Completeness [%]	100.0	100
Redundancy	14.7	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.8M potassium phosphate, 0.8M sodium phosphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.8M potassium phosphate, 0.8M sodium phosphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K