3N4X
Structure of Csm1 full-length
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 105.269, 105.269, 234.792 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.040 - 3.408 |
R-factor | 0.2398 |
Rwork | 0.238 |
R-free | 0.28160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ENTRY ID 3N4R |
RMSD bond length | 0.011 |
RMSD bond angle | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 16731 | |
<I/σ(I)> | 10.5 | 2 |
Completeness [%] | 78.5 | 14 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 150 mM lithium chloride, 12% PEG 2000, 4% 1,4-butanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |