3N4M

E. coli RNA polymerase alpha subunit C-terminal domain in complex with CAP and DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.10
Spacegroup name	P 62 2 2
Unit cell lengths	175.730, 175.730, 160.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.510 - 2.987
R-factor	0.199
Rwork	0.198
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lb2 3k4g
RMSD bond length	0.004
RMSD bond angle	0.946
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	AMoRE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.510	3.180
High resolution limit [Å]	2.987	3.020
Rmerge	0.085	1.106
Number of reflections	29113
<I/σ(I)>	11.5	1.6
Completeness [%]	98.9	99.5
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293.2	100 mM sodium acetate (pH 4.5), 625 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K