3N4J
Putative RNA methyltransferase from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.719, 79.719, 44.638 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.500 - 1.470 |
| R-factor | 0.145 |
| Rwork | 0.143 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mxi |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.049 | 0.811 |
| Number of reflections | 26316 | |
| <I/σ(I)> | 15.5 | 2.91 |
| Completeness [%] | 94.1 | 65 |
| Redundancy | 17.2 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M ammonium sulfate, 0.1 M Tris buffer, 25% PEG MME 5000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
